This episode marks Splendid's record for the highest amount of kills in one episode, counting indirect responsibility (20+ kills: the other 18 main characters if you don't count Flippy, Lammy, Mr. Pickels, Truffles from the Blurb version, and 1+ numerous Generic Tree Friends).
This is the second time Sniffles stars in an episode despite having very little screen time. The first time is in The Wrong Side of the Tracks.
This is the first Happy Tree Friends episode to be produced in 16:9(aspect ratio) for High Definition.
This is the first Season 3 episode where Handy, Lifty, Shifty Mime, Flaky, Splendid and Disco Bear appear.
This episode has a new intro, featuring different character intros and a new ending credit sequence.
This is the second time Petunia has been horizontally sliced in half by Splendid's heat vision. The first time is in From Hero to Eternity.
Flippy, Lammy, and Mr. Pickels are the only main characters who do not appear in this episode (the latter two being more obvious since this was before A Bit of a Pickle). The character in the Rorschach costume was confirmed to be a Generic Tree Friend.
The mask Cub wears resembles what Lumpy looked like in the first ever Happy Tree Friends episode: Banjo Frenzy.
Cro-Marmot has a group of statues closely resembling The Cursed Idol on his booth. The main difference is is that the statues are polished unlike the actual Cursed Idol.
Similar to Swelter Skelter, the Cursed Idol can be seen briefly in the explosion when the convention blows up.
Despite Splendid's high kill count, this is the first time he kills Mime, Disco Bear, and Pop. As of this episode, Flippy, Lammy and Mr. Pickels are the only main characters that Splendid has not killed yet.
This is the only episode where Splendid clearly speaks instead of making different sounds (e.g., hum, gasp, scream, etc.). His voice is also slightly higher than it usually is.
This is the second episode to only have one main character not appear, in this case Flippy. The first time was Asbestos I Can Do, where Cro-Marmot didn't appear.
There are several references to the comic book series Watchmen:
On Sniffles' backpack is badge that resembles a blood-stained smiley face.
A character can be seen dressed as Rorschach, one of the main characters.
Lumpy has Dr. Manhattan's symbol on his forehead. Additionally, Lumpy does not have any pupils, another physical characteristic of Dr. Manhattan.
The last few annotations for this episode's Blurb references the fact that the very first comic created was called "The Adventures of Mr. Obadiah Oldbuck" (or by its Swedish title "Histoire de M. Vieux Bois"), created in 1837 by cartoonist Rodolphe Töpffer. The whole comic can be read on the Dartmouth College website.
The building in which the convention is hosted is a direct parody of the Hall of Justice from the 1970s "Super Friends" cartoons.
Mime, Disco Bear, and Russell's deaths are similar to Nutty, Sniffles, and Mime's deaths in Aw, Shucks!. Coincidentally, Mime is in both groups.
Petunia's death is similar to her death in From Hero to Eternity and Cuddles' injury in Just Be Clause, since all of these involve getting horizontally sliced in half by Splendid's laser.
The Acorn Shield Symbol for the SSSSSuper Squad can be seen numerous times on Sniffles' backpack, in comic books, and on Splendid's desk, making this the first Internet episode to reference the Splendid Ka-Pow! series.
According to the "Revenge Of Con" page, Petunia was originally going to have Giggles' role and Giggles was going to be in Petunia's place, but they swapped places in the final cut for unknown reasons.
According to the storyboards, they were also both originally going to have light-sabers.
According to the episode's beat-sheet, the episode originally ended with the explosion wiping Sniffles out when he entered, causing a huge crater and leaving only Splendid and his podium, which would then crumble.
According to the Revenge of Con blog (no longer available), the convention center building was supposed to be based on the Legion of Doom headquarters.